Collect all the bee items in a stage.
(Can be done in NOVICE mode)
Simply collect all BEES in a level, there are normally 7 BEES per level, but if you're on the secret route there will be 9. I suggest playing on NOVICE and simply collecting all 7 BEES in the first level to unlock the achievement. In NOVICE mode you do not have to uncover the BEES so it's fairly straight forward, all you have to do is move over them to pick them up.